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Hsp70 [SA0379]

Product code: ET1601-11

  • Overview

  • Product name Hsp70
  • Molecular Wt 70 kDa
  • Description The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressedheat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.
  • Species reactivity Human, Mouse, (Predicted: Rat)
  • Tested applications WB,ICC/IF,IHC-P,FC
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen Recombinant protein within human Hsp70 aa 400-641.
  • Positive control A549 cell lysate, MCF-7 cell lysate, HCT116 cell lysate, Hela, MCF-7, human breast carcinoma tissue, human colon carcinoma tissue, human breast tissue, human kidney tissue, human small intestine tissue, mouse testis tissue, mouse brain tissue, mouse prostate tissue.
  • Conjugate Non-conjugated
  • Clone number SA0379
  • Alternative names DnaK type molecular chaperone HSP70 1 antibody
    Epididymis secretory protein Li 103 antibody
    FLJ54303 antibody
    FLJ54370 antibody
    FLJ54392 antibody
    FLJ54408 antibody
    FLJ75127 antibody
    Heat shock 70 kDa protein 1 antibody
    Heat shock 70 kDa protein 1/2 antibody
    Heat shock 70 kDa protein 1A/1B antibody
    Heat shock 70kDa protein 1A antibody
    Heat shock 70kDa protein 1B antibody
    Heat shock induced protein antibody
    HEL S 103 antibody
    HSP70 1 antibody
    HSP70 1B antibody
    HSP70 2 antibody
    HSP70-1/HSP70-2 antibody
    HSP70-1A antibody
    HSP70.1 antibody
    HSP70.1/HSP70.2 antibody
    HSP70I antibody
    HSP71_HUMAN antibody
    HSP72 antibody
    HSPA1 antibody
    HSPA1A antibody
    HSPA1B antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity Protein A affinity purified.
  • Subcellular location Cytoplasm
  • Database links SwissProt: P0DMV9 Human
    SwissProt: P0DMV8 Human
    SwissProt: P17879 Mouse
    SwissProt: Q61696 Mouse
    SwissProt: Q07439 Rat
  • Applications

    WB: 1:500-1:5,000
    ICC/IF: 1:50-1:200
    IHC-P: 1:50-1:200
    FC: 1:50-1:100

  • Publishing research using Hsp70? Please let us know so that we can cite the reference in this datasheet.

  • Yin, K., Guan, Y., Ma, R., Wei, L., Liu, B., Liu, X., . . . Wang, Q. (2018). Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida. PLoS Pathog, 14(8), e1007272. PMID: 30169545

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols
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