PRODUCT CODE: em1701-85

Zscan4 Monoclonal Antibody (EM1701-85)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of Zscan4 on 293 transfected Zscan4 (1) and 293T (2) cell lysates using anti-Zscan4 antibody at 1/500 dilution.
  • Western blot analysis of Zscan4 on 293 transfected Zscan4 (1) and 293T (2) cell lysates using anti-Zscan4 antibody at 1/500 dilution.
  • Flow cytometric analysis of HepG2 cells with Zscan4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of Zscan4 on 293 transfected Zscan4 (1) and 293T (2) cell lysates using anti-Zscan4 antibody at 1/500 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Zscan4 Monoclonal Antibody (EM1701-85)

Immunogen

Recombinant protein within human zscan4 aa 1-100.

Host

Mouse

Positive Control

293 tranfected Zscan4, 293T, HepG2, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

43868

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

49 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Zinc finger and SCAN domain-containing protein 4

GENE NAME

ZSCAN4

SYNONYMS

ZSCAN4, ZNF494

SUBCELLULAR LOCATION

Nucleus. Chromosome, telomere.

FUNCTION

Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells (By similarity).