PRODUCT CODE: ER41204

ZO1 Antibody (ER41204)

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining ZO1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ZO1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZO1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ZO1 antibody. Counter stained with hematoxylin.
ICC staining ZO1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ZO1 Antibody (ER41204)

Immunogen

Peptide

Host

Rabbit

Positive Control

MCF-7, human tonsil tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

ZO1

SYNONYMS

Tight junction protein 1 antibody; Tight junction protein ZO-1 antibody; Tight junction protein ZO1 antibody; TJP1 antibody; zo-1 antibody; Zo1 antibody; ZO1_HUMAN antibody; Zona occludens 1 antibody; Zona occludens 1 protein antibody; Zona occludens protein 1 antibody; Zonula occludens 1 protein antibody; Zonula occludens protein 1 antibody

SEQUENCE SIMILARITIES

Belongs to the MAGUK family.

TISSUE SPECIFICITY

The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ.

SUBCELLULAR LOCATION

Cell membrane, cell junction

FUNCTION

Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.