PRODUCT CODE: ER1706-82

ZBTB48 Antibody (ER1706-82)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ZBTB48 on mouse spleen tissue lysate using anti-ZBTB48 antibody at 1/1,000 dilution.<br />
  • Western blot analysis of ZBTB48 on mouse spleen tissue lysate using anti-ZBTB48 antibody at 1/1,000 dilution.<br />
  • ICC staining ZBTB48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ZBTB48 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with ZBTB48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of ZBTB48 on mouse spleen tissue lysate using anti-ZBTB48 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ZBTB48 Antibody (ER1706-82)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse spleen tissue lysate, HepG2, PC-3M, SH-SY5Y, human liver cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

77 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Telomere zinc finger-associated protein

GENE NAME

ZBTB48

SYNONYMS

TZAP, hKR3, ZBTB48, HKR3, TZAP, ZNF855

SEQUENCE SIMILARITIES

Belongs to the krueppel C2H2-type zinc-finger protein family.

TISSUE SPECIFICITY

Detected in adrenal gland and neuroblastoma.

SUBCELLULAR LOCATION

Nucleus. Note=Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat. According to a report, preferentially binds to long telomeres that have a low concentration of shelterin complex, competing with the telomeric repeat binding factors TERF1 and TERF2. According to another report, binds telomeres regardless of their length.

FUNCTION

Telomere-binding protein that acts as a regulator of telomere length. Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat. Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres. Also acts as a transcription regulator that binds to promoter regions. Regulates expression of a small subset of genes, including MTFP1. Regulates expression the J and/or S elements in MHC II promoter. Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A.