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PRODUCT CODE: EM1902-31

YKL-40/CHI3L1 Monoclonal Antibody (EM1902-31)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

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Western blot analysis of YKL-40/CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of YKL-40/CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YKL-40/CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-31, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of YKL-40/CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

YKL-40/CHI3L1 Monoclonal Antibody (EM1902-31)

Immunogen

Recombinant protein within extracellular domain of human chi3l1.

Host

Mouse

Positive Control

THP-1 cell lysates, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A3G10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • IHC-P:1:50-1:200

TARGET

UNIPROT #

P36222

PROTEIN NAME

YKL-40/CHI3L1

SYNONYMS

39 kDa synovial protein antibody; ASRT7 antibody; Cartilage glycoprotein 39 antibody; CGP-39 antibody; CGP39 antibody; CH3L1_HUMAN antibody; CHI3L1 antibody; chitinase 3 like 1 (cartilage glycoprotein 39) antibody; chitinase 3 like 1 antibody; Chitinase 3 like protein 1 precursor antibody; chitinase antibody; Chitinase-3-like protein 1 antibody; Chondrocyte protein YKL40 antibody; GP 39 antibody; GP-39 antibody; GP39 antibody; HC gp39 antibody; HCGP 3P antibody; hCGP-39 antibody; HCgp39 antibody; YKL 40 antibody; YKL-40 antibody; YKL40 antibody; YYL 40 antibody

SEQUENCE SIMILARITIES

Belongs to the glycosyl hydrolase 18 family.

TISSUE SPECIFICITY

Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

POST-TRANSLATIONAL MODIFICATION

Glycosylated.

SUBCELLULAR LOCATION

Extracellular space, Endoplasmic reticulum, Cytoplasm.

FUNCTION

Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.