PRODUCT CODE: ET7108-32

WSTF Recombinant Rabbit Monoclonal Antibody [JG36-32] (ET7108-32)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of WSTF on SiHa cell lysates using anti-WSTF at 1/500 dilution.
  • Western blot analysis of WSTF on SiHa cell lysates using anti-WSTF at 1/500 dilution.
  • Flow cytometric analysis of K562 cells with WSTF antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of WSTF on SiHa cell lysates using anti-WSTF at 1/500 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

WSTF Recombinant Rabbit Monoclonal Antibody [JG36-32] (ET7108-32)

Immunogen

Recombinant protein within c-terminal human wstf.

Host

Rabbit

Positive Control

SiHa, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG36-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

171 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

WSTF

SYNONYMS

baz1b antibody; BAZ1B_HUMAN antibody; Bromodomain adjacent to zinc finger domain protein 1B antibody; hWALP 2 antibody; hWALP-2 antibody; hWALP2 antibody; transcription factor WSTF antibody; Tyrosine-protein kinase BAZ1B antibody; WALP-2 antibody; WALP2 antibody; WBRS 9 antibody; WBRS-9 antibody; WBRS9 antibody; WBSC 10 antibody; WBSC-10 antibody; WBSC10 antibody; WBSCR10 antibody; WBSCR9 antibody; Williams Beuren syndrome chromosome region 9 protein antibody; Williams syndrome transcription factor antibody; Williams-Beuren syndrome chromosomal region 10 protein antibody; Williams-Beuren syndrome chromosomal region 9 protein antibody; WSTF antibody

SEQUENCE SIMILARITIES

Belongs to the WAL family. BAZ1B subfamily.

TISSUE SPECIFICITY

Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.

DEVELOPMENTAL STAGE

Expressed at equal levels in 19-23 weeks old fetal tissues.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication.