PRODUCT CODE: ER1902-66

WDR70 Antibody (ER1902-66)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of WDR70 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of WDR70 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of WDR70 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-66, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of WDR70 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

WDR70 Antibody (ER1902-66)

Immunogen

Synthetic peptide within human wdr70 aa 1-50.

Host

Rabbit

Positive Control

Hela cell lysates, SiHa.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

Predicted band size: 73 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1,000

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

WDR70

SYNONYMS

4833422F06Rik antibody; FLJ10233 antibody; MGC109151 antibody; RGD1309487 antibody; WD repeat containing protein 70 antibody; WD repeat domain 70 antibody; WD repeat-containing protein 70 antibody; wdr70 antibody; WDR70_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat GAD-1 family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

WDR70 Antibody (C-5) is a high quality monoclonal WDR70 antibody (also designated WDR70 antibody) suitable for the detection of the WDR70 protein of mouse, rat and human origin. WDR70 Antibody (C-5) is available as both the non-conjugated anti-WDR70 antibody form, as well as multiple conjugated forms of anti-WDR70 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids, which commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms involving signal transduction, apoptosis, transcriptional regulation or cell cycle control. WD repeats serve as sites for protein-protein interaction and some seem to mediate the assembly of protein complexes. WDR70 (WD repeat-containing protein 70) is a 654 amino acid protein that contains seven WD repeats and belongs to the WD repeat GAD-1 family. WDR70 is encoded by a gene located on human chromosome 5q13.3.