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PRODUCT CODE: ER1803-16

WASL Antibody (ER1803-16)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

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Western blot analysis of WASL on SK-BR-3 and SH-SY-5Y cell lysate using anti- WASL antibody at 1/500 dilution.
  • Western blot analysis of WASL on SK-BR-3 and SH-SY-5Y cell lysate using anti- WASL antibody at 1/500 dilution.
  • ICC staining WASL in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining WASL in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining WASL in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- WASL antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti- WASL antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with WASL antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of WASL on SK-BR-3 and SH-SY-5Y cell lysate using anti- WASL antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

WASL Antibody (ER1803-16)

Immunogen

Recombinant protein within human wasl aa 100-300.

Host

Rabbit

Positive Control

SH-SY-5Y, SiHa, SK-Br-3, rat brain tissue, human small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

55 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50 FC

TARGET

UNIPROT #

O00401

PROTEIN NAME

WASL

SYNONYMS

WASP family, verprolin homology domain-containing protein 1 antibody; WASP family member 1 antibody; FLJ31482 antibody; homology of dictyostelium scar 1 antibody; KIAA0269 antibody; Protein WAVE-1 antibody; Protein WAVE1 antibody; scar, Dictyostelium, homology of, 1 antibody; SCAR1 antibody; Similar to a plant extensin like protein antibody; Verprolin homology domain containing protein 1 antibody; Verprolin homology domain-containing protein 1 antibody; WAS protein family, member 1 antibody; Wasf1 antibody; WASF1_HUMAN antibody; WASL antibody; WASP family 1 antibody; WASP family protein member 1 antibody; WASP family, verprolin homology domain-containing protein antibody; WAVE antibody; WAVE1 antibody; Wiskott Aldrich syndrome protein family member 1 antibody; Wiskott-Aldrich syndrome protein family member 1 antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity.

SUBCELLULAR LOCATION

Cytoplasm. Cytoskeleton. Nucleus.

FUNCTION

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis.