Von Willebrand Factor Recombinant Rabbit Monoclonal Antibody [PSH07-45] (HA722833)
Catalog# HA722833
Rabbit Monoclonal to Von Willebrand Factor
Tested for
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WB
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IHC-P
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IF-Tissue
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate (negative)
Lane 2: Human lung tissue lysate
Lane 3: Rat lung tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 309 kDa
Observed band size: 309 kDa
Exposure time: Lane 1-3 (left): 25 seconds; Lane 1-3 (right): 3 minutes; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate (negative)
Lane 2: Human lung tissue lysate
Lane 3: Rat lung tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 309 kDa
Observed band size: 309 kDa
Exposure time: Lane 1-3 (left): 25 seconds; Lane 1-3 (right): 3 minutes; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.