PRODUCT CODE: ER1706-99

VEGF Receptor 2 Rabbit Polyclonal Antibody (ER1706-99)

Applications

  • ICC

  • IHC-P

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining VEGF Receptor 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VEGF Receptor 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VEGF Receptor 2 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VEGF Receptor 2 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-VEGF Receptor 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-VEGF Receptor 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-VEGF Receptor 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VEGF Receptor 2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HUVEC cells with VEGF Receptor 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
ICC staining VEGF Receptor 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

VEGF Receptor 2 Rabbit Polyclonal Antibody (ER1706-99)

Immunogen

Synthetic peptide within human vegf receptor 2 aa 1282-1335.

Host

Rabbit

Positive Control

HepG2, HUVEC, PMVEC, rat kidney tissue, human breast tissue, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

152 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • WB

  • 1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

VEGF Receptor 2

SYNONYMS

CD309 antibody; CD309 antigen antibody; EC 2.7.10.1 antibody; Fetal liver kinase 1 antibody; FLK-1 antibody; FLK1 antibody; FLK1, mouse, homolog of antibody; Kdr antibody; Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody; Kinase insert domain receptor antibody; KRD1 antibody; Ly73 antibody; Protein tyrosine kinase receptor FLK1 antibody; Protein-tyrosine kinase receptor flk-1 antibody; soluble VEGFR2 antibody; Tyrosine kinase growth factor receptor antibody; Vascular endothelial growth factor receptor 2 antibody; VEGFR 2 antibody; VEGFR antibody; VEGFR-2 antibody; VEGFR2 antibody; VGFR2_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

TISSUE SPECIFICITY

Detected in cornea (at protein level). Widely expressed.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.; Ubiquitinated. Tyrosine phosphorylation of the receptor promotes its poly-ubiquitination, leading to its degradation via the proteasome or lysosomal proteases.; Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-951 is important for interaction with SH2D2A/TSAD and VEGFA-mediated reorganization of the actin cytoskeleton. Phosphorylation at Tyr-1175 is important for interaction with PLCG1 and SHB. Phosphorylation at Tyr-1214 is important for interaction with NCK1 and FYN. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.; The inhibitory disulfide bond between Cys-1024 and Cys-1045 may serve as a specific molecular switch for H(2)S-induced modification that regulates KDR/VEGFR2 function.

SUBCELLULAR LOCATION

Nucleus. Membrane. Secreted.

FUNCTION

Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1) (1-3), Flk-1 (also designated VEGF-R2) (4-6) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs) (3,5). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.