PRODUCT CODE: ER30603

VCP Antibody (ER30603)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution.<br />
Positive control: <br />
Lane 1: L929 <br />
Lane 2: F9 <br />
Lane 3: A549 <br />
Lane 4: MCF-7 <br />
Lane 5: Hela <br />
Lane 6: NIH/3T3 <br />
Lane 7: N2A <br />
Lane 8: Jurkat <br />
Lane 9: HepG2 <br />
Lane 10: A431 <br />
Lane 11: Mouse heart <br />
 Lane 12: Mouse brain <br />
Lane 13: Mouse liver <br />
Lane 14: Mouse kidney
  • Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution.<br />
Positive control: <br />
Lane 1: L929 <br />
Lane 2: F9 <br />
Lane 3: A549 <br />
Lane 4: MCF-7 <br />
Lane 5: Hela <br />
Lane 6: NIH/3T3 <br />
Lane 7: N2A <br />
Lane 8: Jurkat <br />
Lane 9: HepG2 <br />
Lane 10: A431 <br />
Lane 11: Mouse heart <br />
 Lane 12: Mouse brain <br />
Lane 13: Mouse liver <br />
Lane 14: Mouse kidney
  • ICC staining VCP in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCP in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VCP antibody. Counter stained with hematoxylin.
Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution.
Positive control:
Lane 1: L929
Lane 2: F9
Lane 3: A549
Lane 4: MCF-7
Lane 5: Hela
Lane 6: NIH/3T3
Lane 7: N2A
Lane 8: Jurkat
Lane 9: HepG2
Lane 10: A431
Lane 11: Mouse heart
Lane 12: Mouse brain
Lane 13: Mouse liver
Lane 14: Mouse kidney

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

VCP Antibody (ER30603)

Immunogen

Peptide

Host

Rabbit

Positive Control

L929, F9, SW480, A549, MCF-7, Hela, NIH/3T3, N2A, Jurkat, HepG2, A431,mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue,human uterus tissue,SHG-44 cells ,mouse skeletal muscle tissue .

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

89 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Transitional endoplasmic reticulum ATPase

GENE NAME

VCP

SEQUENCE SIMILARITIES

Belongs to the AAA ATPase family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation. Phosphorylated in mitotic cells.; ISGylated.; Methylation at Lys-315 catalyzed by VCPKMT is increased in the presence of ASPSCR1. Lys-315 methylation may decrease ATPase activity.

SUBCELLULAR LOCATION

Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus. Cytoplasm, Stress granule. Note=Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. Recruited to the cytoplasmic surface of the endoplasmic reticulum via interaction with AMFR/gp78. Following DNA double-strand breaks, recruited to the sites of damage. Recruited to stalled replication forks via interaction with SPRTN. Recruited to damaged lysosomes decorated with K48-linked ubiquitin chains. Colocalizes with TIA1, ZFAND1 and G3BP1 in cytoplasmic stress granules (SGs) in response to arsenite-induced stress treatment.

FUNCTION

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Plays a role in the regulation of stress granules (SGs) clearance process upon arsenite-induced response. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy. Acts as a negative regulator of type I interferon production by interacting with DDX58/RIG-I: interaction takes place when DDX58/RIG-I is ubiquitinated via 'Lys-63'-linked ubiquitin on its CARD domains, leading to recruit RNF125 and promote ubiquitination and degradation of DDX58/RIG-I. May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation. May more particularly play a role in caveolins sorting in cells. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.