PRODUCT CODE: R1512-13

VCAM1 Antibody (R1512-13)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of VCAM1 on human spleen tissue lysate using anti-VCAM1 antibody at 1/5,000 dilution.
  • Western blot analysis of VCAM1 on human spleen tissue lysate using anti-VCAM1 antibody at 1/5,000 dilution.
  • ICC staining VCAM1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCAM1 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCAM1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with VCAM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of VCAM1 on human spleen tissue lysate using anti-VCAM1 antibody at 1/5,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

VCAM1 Antibody (R1512-13)

Immunogen

Peptide

Host

Rabbit

Positive Control

Human spleen tissue lysate, Hela,JAR, SHG-44, human spleen tissue, HUVEC.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Cafter thawing. Aliquot store at -20Cor -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

VCAM1

SYNONYMS

CD106 antibody; CD106 Antigen antibody; INCAM 100 antibody; INCAM-100 antibody; L1CAM antibody; MGC99561 antibody; V-CAM 1 antibody; Vascular Cell Adhesion Molecule 1 antibody; Vascular cell adhesion protein 1 antibody; VCAM 1 antibody; VCAM-1 antibody; VCAM1 antibody; VCAM1_HUMAN antibody

TISSUE SPECIFICITY

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

POST-TRANSLATIONAL MODIFICATION

Sialoglycoprotein.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms' tumors, Ewing's sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2α1 and B2αM integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM) -like protein and is deleted in 66% of patients with Kallmann's syndrome, anosmia with secondary hypogonadism.