PRODUCT CODE: 0905-5

Vang-like protein 1 Antibody (0905-5)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

Western blot analysis of VANGL1 on MCF-7 cell lysate using anti-VANGL1 antibody at 1/500 dilution.
  • Western blot analysis of VANGL1 on MCF-7 cell lysate using anti-VANGL1 antibody at 1/500 dilution.
  • ICC staining VANGL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VANGL1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VANGL1in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of VANGL1 on MCF-7 cell lysate using anti-VANGL1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Vang-like protein 1 Antibody (0905-5)

Immunogen

Synthetic peptide within human 100-160 aa.

Host

Rabbit

Positive Control

Hela, MCF-7, LOVO.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

80 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Vang-like protein 1

SYNONYMS

ANGL planar cell polarity protein 1 antibody; KAI1 C-terminal interacting tetraspanin antibody; KITENIN antibody; Loop tail protein 2 homolog antibody; Loop-tail protein 2 homolog antibody; LPP2 antibody; MGC5338 antibody; STB2 antibody; STBM2 antibody; Strabismus 2 antibody; Strabismus, Drosophila, homolog of, 2 antibody; Van Gogh like protein 1 antibody; Van Gogh, Drosophila, homolog of, 1 antibody; Van Gogh-like protein 1 antibody; Vang like 1 (van gogh, Drosophila) antibody; Vang like protein 1 antibody; Vang-like 1 antibody; Vang-like protein 1 antibody; VANG1_HUMAN antibody; VANGL 1 antibody; Vangl1 antibody

SEQUENCE SIMILARITIES

Belongs to the Vang family.

TISSUE SPECIFICITY

According to PubMed:11956595, ubiquitously expressed. According to PubMed:12011995, expressed specifically in testis and ovary.

SUBCELLULAR LOCATION

Cell membrane. Membrane.

FUNCTION

Vang-like protein 1 is a multi-pass membrane protein, expressed specifically in testis and ovary. It interacts through its C-terminal region with the N-terminal half of DVL1, DVL2 and DVL3. Vangl proteins play a key developmental role in establishing planar cell polarity (PCP) and in regulating convergent extension (CE) movements during embryogenesis. Vangl1 and Vangl2 are both downregulated in several cancer cell lines and primary tumors. Defects in VANGL1 are a cause of neural tube defects (NTD) and a cause of sacral defect with anterior meningocele (SDAM).