Positive control:
Lane 1: THP-1 cell lysate
Lane 2: Rat testis tissue lysate
Applications
-
WB
-
FC
REACTIVITY
-
Human
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
USP29 Antibody (ER1902-76)
Immunogen
Recombinant protein within human usp29 aa 665-942.
Host
Rabbit
Positive Control
THP-1 cell lysate, rat testis tissue lysate, 293.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
104 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
USP29
SYNONYMS
Deubiquitinating enzyme 29 antibody; HOM TES 84/86 antibody; MGC163266 antibody; MGC163270 antibody; Ubiquitin carboxyl terminal hydrolase 29 antibody; Ubiquitin carboxyl-terminal hydrolase 29 antibody; Ubiquitin specific peptidase 29 antibody; Ubiquitin specific processing protease 29 antibody; Ubiquitin specific processing protease antibody; Ubiquitin thiolesterase 29 antibody; Ubiquitin-specific-processing protease 29 antibody; UBP29_HUMAN antibody; USP 29 antibody; Usp29 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C19 family.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The DNA damage checkpoint is essential for the maintenance of genome integrity after genotoxic stress, and also for cell survival in eukaryotes. Claspin has a key role in the ATR (ATM and Rad3-related)-Chk1 branch of the DNA damage checkpoint and is also required for correct DNA replication. To achieve properly these functions, Claspin is tightly regulated by ubiquitinin-dependent proteasomal degradation, which controls Claspin levels in a DNA-damage- and cell-cycle-dependent manner. USP29 is a regulator of Claspin, USP29 is a player in the ATR-Chk1 pathway and the control of DNA replication.