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PRODUCT CODE: ER1902-76

USP29 Antibody (ER1902-76)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Rat

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Western blot analysis of USP29 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: THP-1 cell lysate<br /> Lane 2: Rat testis tissue lysate
  • Western blot analysis of USP29 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: THP-1 cell lysate<br /> Lane 2: Rat testis tissue lysate
  • Flow cytometric analysis of USP29 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-76, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; blcak).
Western blot analysis of USP29 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: Rat testis tissue lysate

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

USP29 Antibody (ER1902-76)

Immunogen

Recombinant protein within human usp29 aa 665-942.

Host

Rabbit

Positive Control

THP-1 cell lysate, rat testis tissue lysate, 293.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

104 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q9HBJ7

PROTEIN NAME

USP29

SYNONYMS

Deubiquitinating enzyme 29 antibody; HOM TES 84/86 antibody; MGC163266 antibody; MGC163270 antibody; Ubiquitin carboxyl terminal hydrolase 29 antibody; Ubiquitin carboxyl-terminal hydrolase 29 antibody; Ubiquitin specific peptidase 29 antibody; Ubiquitin specific processing protease 29 antibody; Ubiquitin specific processing protease antibody; Ubiquitin thiolesterase 29 antibody; Ubiquitin-specific-processing protease 29 antibody; UBP29_HUMAN antibody; USP 29 antibody; Usp29 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The DNA damage checkpoint is essential for the maintenance of genome integrity after genotoxic stress, and also for cell survival in eukaryotes. Claspin has a key role in the ATR (ATM and Rad3-related)-Chk1 branch of the DNA damage checkpoint and is also required for correct DNA replication. To achieve properly these functions, Claspin is tightly regulated by ubiquitinin-dependent proteasomal degradation, which controls Claspin levels in a DNA-damage- and cell-cycle-dependent manner. USP29 is a regulator of Claspin, USP29 is a player in the ATR-Chk1 pathway and the control of DNA replication.