PRODUCT CODE: ER1802-25

USP21 Antibody (ER1802-25)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

  • Mouse

Western blot analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution.
  • Western blot analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution.
  • ICC staining USP21 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP21 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-USP21 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-USP21 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-USP21 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with USP21 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

USP21 Antibody (ER1802-25)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse thymus tissue lysate, PC-3M, SH-SY-5Y, human liver cancer tissue, human kidney tissue, human fetal skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

63 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Ubiquitin carboxyl-terminal hydrolase 21

GENE NAME

USP21

SYNONYMS

USP21, USP23, PP1490

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family. USP21 subfamily.

TISSUE SPECIFICITY

Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Deubiquitinates BAZ2A/TIP5 leading to its stabilization.