PRODUCT CODE: ER1803-55

USP14 Antibody (ER1803-55)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of USP14 on Daudi cell lysate using anti-USP14 antibody at 1/500 dilution.
  • Western blot analysis of USP14 on Daudi cell lysate using anti-USP14 antibody at 1/500 dilution.
  • Flow cytometric analysis of SiHa cells with USP14 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody;black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of USP14 on Daudi cell lysate using anti-USP14 antibody at 1/500 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

USP14 Antibody (ER1803-55)

Immunogen

Recombinant protein within human usp14 aa 200-450.

Host

Rabbit

Positive Control

Daudi, SiHa.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

56 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Ubiquitin carboxyl-terminal hydrolase 14

GENE NAME

USP14

SYNONYMS

USP14, TGT

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

SUBCELLULAR LOCATION

Cytoplasm. Cell membrane; Peripheral membrane protein.

FUNCTION

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.