PRODUCT CODE: ER1803-09

USP13 Antibody (ER1803-09)

Applications

  • WB

  • ICC

  • FC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: 293T <br />
  Lane 2: Daudi<br />
  Lane 3: Mouse testis <br />
  Lane 4: Rat brain
  • Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: 293T <br />
  Lane 2: Daudi<br />
  Lane 3: Mouse testis <br />
  Lane 4: Rat brain
  • ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Daudi
Lane 3: Mouse testis
Lane 4: Rat brain

Applications

  • WB

  • ICC

  • FC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

USP13 Antibody (ER1803-09)

Immunogen

Recombinant protein within human usp13 aa 550-800.

Host

Rabbit

Positive Control

293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

97 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

USP13

SYNONYMS

Deubiquitinating enzyme 13 antibody; Isopeptidase T 3 antibody; Isopeptidase T-3 antibody; Isopeptidase T3 antibody; ISOT 3 antibody; ISOT-3 antibody; ISOT3 antibody; Ubiquitin carboxyl terminal hydrolase 13 antibody; Ubiquitin carboxyl-terminal hydrolase 13 antibody; Ubiquitin specific peptidase 13 (isopeptidase T3) antibody; Ubiquitin specific peptidase 13 antibody; Ubiquitin specific processing protease 13 antibody; Ubiquitin specific protease 13 antibody; Ubiquitin thioesterase 13 antibody; Ubiquitin thiolesterase 13 antibody; Ubiquitin-specific-processing protease 13 antibody; UBP13 antibody; UBP13_HUMAN antibody; USP 13 antibody; USP13 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family.

TISSUE SPECIFICITY

Highly expressed in ovary and testes.

SUBCELLULAR LOCATION

Nucleus. Cytosol.

FUNCTION

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.