PRODUCT CODE: ER31212

Ubiquitin Antibody (ER31212)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Ubiquitin on different cell lysates using anti-Ubiquitin antibody at 1/1000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: HepG2 <br />
 Lane 3: Hela <br />
 Lane 4: MCF-2
  • Western blot analysis of Ubiquitin on different cell lysates using anti-Ubiquitin antibody at 1/1000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: HepG2 <br />
 Lane 3: Hela <br />
 Lane 4: MCF-2
  • ICC staining Ubiquitin in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ubiquitin in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ubiquitin in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Ubiquitin antibody. Counter stained with hematoxylin.
Western blot analysis of Ubiquitin on different cell lysates using anti-Ubiquitin antibody at 1/1000 dilution.
Positive control:
Lane 1: 293T
Lane 2: HepG2
Lane 3: Hela
Lane 4: MCF-2

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Ubiquitin Antibody (ER31212)

Immunogen

Peptide

Host

Rabbit

Positive Control

293T, HepG2, Hela, MCF-7, human tonsil tissue,mouse spleen tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

10 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Ubiquitin

SYNONYMS

Epididymis secretory protein Li 50 antibody; FLJ25987 antibody; HEL S 50 antibody; MGC8385 antibody; Polyubiquitin B antibody; RPS 27A antibody; RPS27A antibody; UBA 52 antibody; UBA 80 antibody; UBA52 antibody; UBA80 antibody; UBB antibody; UBB_HUMAN antibody; UBC antibody; UBCEP 1 antibody; UBCEP 2 antibody; UBCEP1 antibody; UBCEP2 antibody; Ubiquitin antibody; Ubiquitin B antibody

SEQUENCE SIMILARITIES

Belongs to the ubiquitin family.

POST-TRANSLATIONAL MODIFICATION

[Ubiquitin]: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30.; [Ubiquitin]: Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.

SUBCELLULAR LOCATION

Cytoplasm, nucleus

FUNCTION

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B.