PRODUCT CODE: em1701-50

UAP1 Monoclonal Antibody (EM1701-50)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of UAP1 on Jurkat cell lysate using anti-UAP1 antibody at 1/500 dilution.<br />
  • Western blot analysis of UAP1 on Jurkat cell lysate using anti-UAP1 antibody at 1/500 dilution.<br />
  • ICC staining UAP1 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining UAP1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
Western blot analysis of UAP1 on Jurkat cell lysate using anti-UAP1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

UAP1 Monoclonal Antibody (EM1701-50)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Jurkat, A549, Hela, rat epididymis tissue, human lung cancer tissue, human colon cancer tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

4H1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

58 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

UDP-N-acetylhexosamine pyrophosphorylase

GENE NAME

UAP1

SYNONYMS

AGX, UAP1, SPAG2

SEQUENCE SIMILARITIES

Belongs to the UDPGP type 1 family.

TISSUE SPECIFICITY

Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver.

SUBCELLULAR LOCATION

Cytoplasm. Note=In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail.

FUNCTION

Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.