PRODUCT CODE: R1706-4

Tyrosinase Antibody (R1706-4)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

-
+
Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.
  • Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.
  • ICC staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tyrosinase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human urethral melanoma tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with Tyrosinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Tyrosinase Antibody (R1706-4)

Immunogen

Peptide

Host

Rabbit

Positive Control

B16F1, Hela, human urethral melanoma tissue, A431.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:200-1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Tyrosinase

SYNONYMS

ATN antibody; CMM8 antibody; LB24 AB antibody; LB24-AB antibody; Monophenol monooxygenase antibody; OCA1 antibody; OCA1A antibody; OCAIA antibody; Oculocutaneous albinism IA antibody; SHEP3 antibody; SK29 AB antibody; SK29-AB antibody; Tumor rejection antigen AB antibody; TYR antibody; TYRO_HUMAN antibody; tyrosinase (oculocutaneous albinism IA) antibody; Tyrosinase antibody

SEQUENCE SIMILARITIES

Belongs to the tyrosinase family.

POST-TRANSLATIONAL MODIFICATION

Glycosylated.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.