PRODUCT CODE: ER1803-05

Tyk2 Antibody (ER1803-05)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TYK2 on MCF-7 cell lysate using anti-TYK2 antibody at 1/500 dilution.
  • Western blot analysis of TYK2 on MCF-7 cell lysate using anti-TYK2 antibody at 1/500 dilution.
  • ICC staining TYK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TYK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- TYK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- TYK2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with TYK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of TYK2 on MCF-7 cell lysate using anti-TYK2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Tyk2 Antibody (ER1803-05)

Immunogen

Recombinant protein within human tyk2 aa 400-600.

Host

Rabbit

Positive Control

MCF-7, rat kidney tissue, human liver cancer tissue, human colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

134 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tyk2

SYNONYMS

JTK 1 antibody; JTK1 antibody; Non receptor tyrosine protein kinase 2 antibody; Non receptor tyrosine protein kinase TYK2 antibody; Non-receptor tyrosine-protein kinase TYK2 antibody; OTTHUMP00000232745 antibody; OTTHUMP00000232746 antibody; OTTHUMP00000232748 antibody; Protein Tyrosine Kinase 2 antibody; TYK 2 antibody; Tyk2 antibody; TYK2_HUMAN antibody; Tyrosine kinase 2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

TISSUE SPECIFICITY

Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.

SUBCELLULAR LOCATION

Cytoskeleton. Nucleus. Cytosol. Plasma membrane.

FUNCTION

TYK2 belongs to the family of non-receptor janus tyrosine kinases, which regulate a spectrum of cellular functions occurring downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, recruit STAT transcription factors to the cytokine receptor where TYK2 is associated. TYK2 then phosphorylates proximal STAT factors, which subsequently dimerize, translocate to the nucleus, and bind to cis elements upstream of target gene promoters to regulating transcription. The canonical JAK-STAT pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Cytokine induced pro-inflammatory responses are attractive targets for anti-inflammatory therapies, specifically at the level of Jak-STAT signaling.