PRODUCT CODE: R1510-2

TSC2/Tuberin Antibody (R1510-2)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Immunocytochemical staining of SH-SY-5Y cells using anti-Tuberin rabbit polyclonal antibody.
  • Immunocytochemical staining of SH-SY-5Y cells using anti-Tuberin rabbit polyclonal antibody.
  • Immunocytochemical staining of PANC-1 cells using anti-Tuberin rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human pancreas tissue using anti-Tuberin rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse liver tissue using anti-Tuberin rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-Tuberin rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse pancreas tissue using anti-Tuberin rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-Tuberin rabbit polyclonal antibody.
  • Flow cytometric analysis of Hela cells with Tuberin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.
Immunocytochemical staining of SH-SY-5Y cells using anti-Tuberin rabbit polyclonal antibody.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TSC2/Tuberin Antibody (R1510-2)

Immunogen

Peptide

Host

Rabbit

Positive Control

SH-SY-5Y , PANC-1, human liver tissue, mouse brain tissue, human pancreas tissue, mouse pancreas tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

TSC2/Tuberin

SYNONYMS

FLJ43106 antibody; LAM antibody; OTTHUMP00000158940 antibody; OTTHUMP00000198394 antibody; OTTHUMP00000198395 antibody; PPP1R160 antibody; Protein phosphatase 1, regulatory subunit 160 antibody; tsc2 antibody; TSC2_HUMAN antibody; TSC4 antibody; TSC4 gene, formerly antibody; TSC4, formerly antibody; Tuberin antibody; Tuberous sclerosis 2 antibody; Tuberous sclerosis 2 protein antibody; Tuberous sclerosis 2 protein homolog antibody

TISSUE SPECIFICITY

Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation. Phosphorylation by AMPK activates it and leads to negatively regulates the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1; phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1.; Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation; association with TSC1 protects from ubiquitination.

SUBCELLULAR LOCATION

Cytoplasm, Membrane

FUNCTION

Tuberous sclerosis complex (TSC) is an autosomal dominant genetic disorder characterized by mental retardation and the widespread development of distinctive tumors termed hamartomas. Two different genetic loci have been linked to TSC; one of these loci, the tuberous sclerosis-2 gene (TSC2), encodes a protein called tuberin and the other loci, tuberous sclerosis-1 gene (TSC1), encodes a protein called hamartin. Tuberin and hamartin interact with each other forming a cystoplasmic complex. Hamartin interacts with the ezrin-radixin-moesin (ERM) family of actin-binding proteins and inhibition of hamartin activity results in loss of cell adhesion. Hamartin is present in most adult tissues with strong expression in brain, heart, and kidney.