Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] (ET1610-37)
Catalog# ET1610-37
Rabbit Monoclonal to Tryptophan Hydroxylase 1 (TPH1)
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
IP
-
FC
-
Human
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Mouse
-
Rat
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Cynomolgus monkey
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Pig
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: Hela cell lysate
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] (ET1610-37)
Immunogen
Synthetic peptide within human tph1 aa 372-419 / 444.
Host
Rabbit
Positive Control
HL-60 cell lysate, Hela cell lysate, rat brain tissue, mouse cerebellum tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC53-07
RRID
APPLICATION DILUTION
-
WB
-
1:500
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
-
IP
-
Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
51 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Tryptophan Hydroxylase 1 (TPH1)
SYNONYMS
Indoleacetic acid 5 hydroxylase antibody;L tryptophan hydroxylase antibody;MGC119994 antibody;TPH 1 antibody;TPH antibody;TPH1 antibody;TPH1_HUMAN antibody;TPRH antibody;TRPH antibody;Tryptophan 5 hydroxylase 1 antibody;Tryptophan 5 monooxygenase 1 antibody;Tryptophan 5 monooxygenase antibody;Tryptophan 5-hydroxylase 1 antibody;Tryptophan 5-monooxygenase 1 antibody;Tryptophan hydroxylase 1 antibody
SEQUENCE SIMILARITIES
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
TISSUE SPECIFICITY
Isoform 2 seems to be less widely expressed than isoform 1.
SUBCELLULAR LOCATION
Cytosol, neuron projection.
FUNCTION
Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland. Alteration of TPH function may be a key factor in the pathology of several neuropsychiatric disorders associated with serotonin, including depression, aggression, alcoholism and schizophrenia. For instance, L-DOPA, which is used as a common therapy for Parkinson’s disease (PD) patients, inhibits TPH function, which subsequently, is thought to contribute to the onset of depression in PD patients.
