PRODUCT CODE: ET1608-44

TrkA Recombinant Rabbit Monoclonal Antibody [JJ084-04] (ET1608-44)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of TrkA on different cell lysates using anti-TrkA antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Mouse brain     <br />
Lane 2: SH-SY-5Y
  • Western blot analysis of TrkA on different cell lysates using anti-TrkA antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Mouse brain     <br />
Lane 2: SH-SY-5Y
  • Western blot analysis of TrkA on N2A cell  lysates using anti-TrkA antibody at 1/500 dilution.
  • ICC staining TrkA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TrkA in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TrkA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-TrkA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TrkA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TrkA antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with TrkA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of TrkA on different cell lysates using anti-TrkA antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse brain
Lane 2: SH-SY-5Y

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

TrkA Recombinant Rabbit Monoclonal Antibody [JJ084-04] (ET1608-44)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

N2A, SH-SY-5Y, Hela, human tonsil tissue, mouse liver tissue, mouse brain tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ084-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

145 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

TrkA

SYNONYMS

gp140trk antibody; High affinity nerve growth factor receptor antibody; High affinity nerve growth factor receptor precursor antibody; MTC antibody; Neurotrophic tyrosine kinase receptor type 1 antibody; NTRK1 antibody; NTRK1_HUMAN antibody; Oncogene TRK antibody; p14-TrkA antibody; p140 TrkA antibody; p140-TrkA antibody; Slow nerve growth antibody; Trk A antibody; TRK antibody; Trk-A antibody; TRK1 antibody; TRK1-transforming tyrosine kinase protein antibody; Tropomyosin-related kinase A antibody; Tyrosine kinase receptor A antibody; Tyrosine kinase receptor antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

TISSUE SPECIFICITY

Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.

POST-TRANSLATIONAL MODIFICATION

Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.; N-glycosylated. Isoform TrkA-I and isoform TrkA-II are N-glycosylated.; Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination by NEDD4L leads to degradation. Ubiquitination regulates the internalization of the receptor (By similarity).

SUBCELLULAR LOCATION

Cell membrane, Early endosome membrane, Late endosome membrane.

FUNCTION

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning. In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system. Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas. Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest.