PRODUCT CODE: ER40124

Topoisomerase II alpha Antibody (ER40124)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution.<br />
 Positive control: <br />
  Lane 1: Jurkat <br />
  Lane 2: NIH/3T3 <br />
  Lane 3: A431 <br />
  Lane 4: L929 <br />
  Lane 5: A549 <br />
  Lane 6: Human liver <br />
  Lane 7: HUVEC
  • Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution.<br />
 Positive control: <br />
  Lane 1: Jurkat <br />
  Lane 2: NIH/3T3 <br />
  Lane 3: A431 <br />
  Lane 4: L929 <br />
  Lane 5: A549 <br />
  Lane 6: Human liver <br />
  Lane 7: HUVEC
  • ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TOP2A in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: NIH/3T3
Lane 3: A431
Lane 4: L929
Lane 5: A549
Lane 6: Human liver
Lane 7: HUVEC

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Topoisomerase II alpha Antibody (ER40124)

Immunogen

Peptide

Host

Rabbit

Positive Control

A549, Hela, NIH/3T3, Jurkat, L929, A431, HUVEC, human liver tissue, human tonsil tissue, human breast cancer tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

174 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Topoisomerase II alpha

SYNONYMS

alpha isozyme antibody; ATP hydrolyzing DNA topoisomerase II alfa antibody; DNA gyrase antibody; DNA topoisomerase (ATP hydrolyzing) antibody; DNA topoisomerase 2 alpha antibody; DNA topoisomerase 2-alpha antibody; DNA topoisomerase II 170 kD antibody; DNA topoisomerase II alpha isozyme antibody; DNA topoisomerase II antibody; DNA Topoisomerase2 antibody; TOP 2A antibody; TOP2 antibody; TOP2A antibody; TOP2A_HUMAN antibody; Topoisomerase DNA II alpha 170kDa antibody; TP2A antibody

SEQUENCE SIMILARITIES

Belongs to the type II topoisomerase family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

SUBCELLULAR LOCATION

Cytoplasm, nucleus.

FUNCTION

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. It is essential during mitosis and meiosis for proper segregation of daughter chromosomes.