PRODUCT CODE: M1302-1

TNNT2 Mouse Monoclonal Antibody [5-C7] (M1302-1)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of TNNT2 on human heart tissue lysate using anti-TNNT2 antibody at 1/2,000 dilution.
  • Western blot analysis of TNNT2 on human heart tissue lysate using anti-TNNT2 antibody at 1/2,000 dilution.
  • ICC staining TNNT2 (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SK-Br-3 cells with TNNT2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of TNNT2 on human heart tissue lysate using anti-TNNT2 antibody at 1/2,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

TNNT2 Mouse Monoclonal Antibody [5-C7] (M1302-1)

Immunogen

Synthetic peptide within human tnnt2 50-100 aa.

Host

Mouse

Positive Control

Human heart tissue lysate, SK-Br-3, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

5-C7

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

36 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

TNNT2

SYNONYMS

Cardiac muscle troponin T antibody; Cardiomyopathy dilated 1D (autosomal dominant) antibody; Cardiomyopathy hypertrophic 2 antibody; CMD1D antibody; CMH2 antibody; CMPD2 antibody; cTnT antibody; LVNC6 antibody; MGC3889 antibody; OTTHUMP00000033864 antibody; OTTHUMP00000033865 antibody; OTTHUMP00000033866 antibody; OTTHUMP00000033867 antibody; OTTHUMP00000033870 antibody; OTTHUMP00000218095 antibody; RCM3 antibody; TNNT 2 antibody; TNNT2 antibody; TNNT2_HUMAN antibody; TnTc antibody; Troponin T cardiac muscle antibody; Troponin T type 2 (cardiac) antibody; Troponin T type 2 cardiac antibody; Troponin T, cardiac muscle antibody; Troponin T2 cardiac antibody

SEQUENCE SIMILARITIES

Belongs to the troponin T family.

TISSUE SPECIFICITY

Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

SUBCELLULAR LOCATION

Cytoskeleton. Cytosol.

FUNCTION

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI).Troponin T type 2 (cTnT,TNNT2) is a cardiac troponin T isoform expressed in the human heart, that is essential for calcium-regulated myofibrillar ATPase activity. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury.