PRODUCT CODE: ER30606

TLR7 Antibody (ER30606)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TLR7 on different cell lysates using anti-TLR7 antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: Raji <br />
Lane 2: Daudi <br />
Lane 3: Jurkat <br />
Lane 4: THP-1
  • Western blot analysis of TLR7 on different cell lysates using anti-TLR7 antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: Raji <br />
Lane 2: Daudi <br />
Lane 3: Jurkat <br />
Lane 4: THP-1
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TLR7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-TLR7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-TLR7 antibody. Counter stained with hematoxylin.
Western blot analysis of TLR7 on different cell lysates using anti-TLR7 antibody at 1/1000 dilution.
Positive control:
Lane 1: Raji
Lane 2: Daudi
Lane 3: Jurkat
Lane 4: THP-1

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TLR7 Antibody (ER30606)

Immunogen

Peptide

Host

Rabbit

Positive Control

Raji, Daudi, Jurkat, THP-1, human tonsil tissue, human lung tissue, mouse lung tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

140 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Toll-like receptor 7

GENE NAME

TLR7

SYNONYMS

TLR7

SEQUENCE SIMILARITIES

Belongs to the Toll-like receptor family.

TISSUE SPECIFICITY

Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome. Lysosome. Cytoplasmic vesicle, phagosome. Note=Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.

FUNCTION

Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce proinflammatory cytokines and interferons, respectively.