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PRODUCT CODE: ER1706-43

TLR4 Antibody (ER1706-43)

Applications

  • ICC

  • IHC-P

  • FC

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

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ICC staining TLR4 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TLR4 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TLR4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TLR4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TLR4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TLR4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-TLR4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TLR4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with TLR4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
ICC staining TLR4 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • FC

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TLR4 Antibody (ER1706-43)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, HUVEC, PMVEC, rat kidney tissue, human skin tissue, human spleen tissue, mouse colon tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

96/91/73 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

O00206

PROTEIN NAME

TLR4

SYNONYMS

ARMD10 antibody; CD284 antibody; CD284 antigen antibody; Homolog of Drosophila toll antibody; hToll antibody; TLR 4 antibody; TLR4 antibody; TLR4_HUMAN antibody; TOLL antibody; Toll like receptor 4 antibody; Toll-like receptor 4 antibody

SEQUENCE SIMILARITIES

Belongs to the Toll-like receptor family.

TISSUE SPECIFICITY

Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.; Phosphorylated on tyrosine residues by LYN after binding lipopolysaccharide.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Six human homologs of the Drosophila Toll receptor were initially identified based on their sequence similarities and designated toll-like receptors (TLR). Toll receptors are involved in mediating dorsoventral polarization in the developing Drosophila embryo and participate in the host immunity. The TLR family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll / IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. Expression of TLR receptors is highest in peripheral blood leukocytes, macro-phages, and TLR6 is highly homologous to TLR1, sharing greater than 65% sequence identity, and, like other members of TLR family, it induces NFkB signaling upon activation.