PRODUCT CODE: ER1802-58

Tle6 Antibody (ER1802-58)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse ovary tissue <br />
  Lane 2: HepG2
  • Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse ovary tissue <br />
  Lane 2: HepG2
  • ICC staining Tle6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tle6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tle6 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Tle6 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Daudi cells with Tle6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse ovary tissue
Lane 2: HepG2

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Tle6 Antibody (ER1802-58)

Immunogen

Synthetic peptide within mouse tle6 aa 150-200.

Host

Rabbit

Positive Control

Mouse ovary tissue lysate, HepG2, A431, A549, SK-Br-3, mouse small intestine tissue, Daudi.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

63 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tle6

SEQUENCE SIMILARITIES

Belongs to the WD repeat Groucho/TLE family.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

The Notch signaling pathway controls various cellular interactions that are important for the specification of a variety of fates in both vertebrates and invertebrates. Key players in the Notch pathway are the TLE genes (for transducin-like enhancer of split, also designated ESG for enhancer of split groucho), which are human homologs of the Drosophila groucho gene. TLE6 (transducin-like enhancer of split 6), also known as GRG6, is a 449 amino acid cytoplasmic protein belonging to the WD repeat groucho/TLE family. As a member of the subcortical maternal complex (SCMC), TLE6 is essential for zygotes to progress beyond the first embryonic cell divisions. TLE6 contains seven WD repeats, a motif known to mediate protein-protein interactions. The WD40 repeat family of proteins is suggested to be involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation.