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PRODUCT CODE: EM1701-20

Tim3 Monoclonal Antibody (EM1701-20)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of tim3 on 293T cell lysate using anti-Tim3 antibody at 1/500 dilution.
  • Western blot analysis of tim3 on 293T cell lysate using anti-Tim3 antibody at 1/500 dilution.
  • ICC staining Tim3 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tim3 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Tim3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Tim3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tim3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Daudi cells with Tim3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of tim3 on 293T cell lysate using anti-Tim3 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Tim3 Monoclonal Antibody (EM1701-20)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

293T, Hela, human tonsil tisssue, human lung cancer tissue, human spleen tissue, Duudi.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1E5

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

33 kDa (Predicted band size)

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q8TDQ0

PROTEIN NAME

Tim3

SYNONYMS

CD366 antibody; FLJ14428 antibody; HAVcr-2 antibody; Havcr2 antibody; HAVR2_HUMAN antibody; Hepatitis A virus cellular receptor 2 antibody; Kidney injury molecule 3 antibody; KIM 3 antibody; KIM3 antibody; T cell immunoglobulin and mucin domain containing 3 antibody; T cell immunoglobulin mucin 3 antibody; T-cell immunoglobulin and mucin domain-containing protein 3 antibody; T-cell immunoglobulin mucin family member 3 antibody; T-cell immunoglobulin mucin receptor 3 antibody; T-cell membrane protein 3 antibody; Tim 3 antibody; TIM-3 antibody; TIM3 antibody; TIMD-3 antibody; TIMD3 antibody

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. TIM family.

TISSUE SPECIFICITY

Expressed in T-helper type 1 (Th1) lymphocytes. Expressed on regulatory T (Treg) cells after TCR stimulation. Expressed in dendritic cells and natural killer (NK) cells. Expressed in epithelial tissues. Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. In progressive HIV-1 infection, expression is up-regulated on HIV-1-specific CD8 T-cells.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated with core 1 or possibly core 8 glycans.; Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9.

SUBCELLULAR LOCATION

Membrane. Cell junction.

FUNCTION

Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse.