PRODUCT CODE: EM1701-93

TGG2 Monoclonal Antibody (EM1701-93)

Applications

  • WB

  • IHC-P

  • IF

REACTIVITY

  • Arabidopsis thaliana

Western blot analysis of TGG2 on Arabidopsis thaliana lysates.<br />
  Positive control:<br />
  Lane 1: Anti-TGG2 antibody, 1/1,000 dilution.<br />
  Lane 2: Anti-TGG2 antibody, preincubated with the control peptide antigen.
  • Western blot analysis of TGG2 on Arabidopsis thaliana lysates.<br />
  Positive control:<br />
  Lane 1: Anti-TGG2 antibody, 1/1,000 dilution.<br />
  Lane 2: Anti-TGG2 antibody, preincubated with the control peptide antigen.
  • IF staining TGG2 (green) in Arabidopsis thaliana (longitudinal section). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • IF staining TGG2 (green) in Arabidopsis thaliana (cross-section). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded Arabidopsis thaliana tissue using anti-TGG2 antibody. Counter stained with hematoxylin.
Western blot analysis of TGG2 on Arabidopsis thaliana lysates.
Positive control:
Lane 1: Anti-TGG2 antibody, 1/1,000 dilution.
Lane 2: Anti-TGG2 antibody, preincubated with the control peptide antigen.

Applications

  • WB

  • IHC-P

  • IF

REACTIVITY

  • Arabidopsis thaliana

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

TGG2 Monoclonal Antibody (EM1701-93)

Immunogen

Synthetic peptide within arabidopsis thaliana aa 350-400.

Host

Mouse

Positive Control

Arabidopsis thaliana.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

6H1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

ProG purified.

MOLECULAR WEIGHT

63kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:200

  • IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

TGG2

SEQUENCE SIMILARITIES

Belongs to the glycosyl hydrolase 1 family.

TISSUE SPECIFICITY

Expressed in phloem-associated cells.

SUBCELLULAR LOCATION

Chloroplast. Cytosol.Secreted.

FUNCTION

May degrade glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones. These toxic degradation products can deter insect herbivores. Seems to function in abscisic acid (ABA) and methyl jasmonate (MeJA) signaling in guard cells. Functionally redundant with TGG1.