PRODUCT CODE: ER1706-98

TEAD4 Antibody (ER1706-98)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of TEAD4 on different lysates using anti-TEAD4 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Hela <br />
 Lane 2: Human lung tissue<br />
 Lane 3: Human placenta tissue <br />
 Lane 4: HepG2 <br />
  • Western blot analysis of TEAD4 on different lysates using anti-TEAD4 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Hela <br />
 Lane 2: Human lung tissue<br />
 Lane 3: Human placenta tissue <br />
 Lane 4: HepG2 <br />
  •  Flow cytometric analysis of LOVO cells with TEAD4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of TEAD4 on different lysates using anti-TEAD4 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Human lung tissue
Lane 3: Human placenta tissue
Lane 4: HepG2

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TEAD4 Antibody (ER1706-98)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, human lung tissue lysate, human placenta tissue lysate, HepG2, LOVO.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Transcriptional enhancer factor TEF-3

GENE NAME

TEAD4

SYNONYMS

TEAD-4, TEAD4, RTEF1, TCF13L1, TEF3

TISSUE SPECIFICITY

Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.