PRODUCT CODE: 0807-4

TdT Antibody (0807-4)

Applications

  • WB

REACTIVITY

  • Human

Western blot analysis on Jurkat using anti-TdT polyclonal antibody.
  • Western blot analysis on Jurkat using anti-TdT polyclonal antibody.
Western blot analysis on Jurkat using anti-TdT polyclonal antibody.

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TdT Antibody (0807-4)

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (klh-coupled) corresponding to near c-terminal residues of human tdt.

Host

Rabbit

Positive Control

Jurkat

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

58kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

TdT

SYNONYMS

Deoxynucleotidyltransferase terminal antibody; DNA nucleotidylexotransferase antibody; DNTT antibody; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody; TDT antibody; TDT_HUMAN antibody; Terminal addition enzyme antibody; Terminal deoxynucleotidyltransferase antibody; Terminal deoxyribonucleotidyltransferase antibody; Terminal transferase antibody

SEQUENCE SIMILARITIES

Belongs to the DNA polymerase type-X family.

SUBCELLULAR LOCATION

Cell nucleus

FUNCTION

Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase expressed in immature lymphocytes of the thymus and bone marrow, as well as certain leukemic cells. The nuclear enzyme TdT plays a critical role in generating immune receptor diversity. During rearrangement of Ig and TCR V region genes, TdT catalyzes the addition of N nucleotides without a DNA template to V-D and D-J junctions in both Ig and TCR as well as the V-J junctions in the TCR (1–3). During the fetal and newborn period, TdT activity is absent, resulting in an N region-deficient Ag receptor repertoire significantly lacking in the diversity observed in the junctional regions of Igs and TCRs of adult animals