Positive control:
Lane 1: K562 cell lysate
Lane 2: Mouse brain tissue lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
SynGAP Antibody (ER1902-57)
Immunogen
Recombinant protein within human syngap aa 1100-1250.
Host
Rabbit
Positive Control
K562 cell lysate, mouse brain tissue lysate, human terus tissue, mouse brain tissue, SW620.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
148 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:100
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
SynGAP
SYNONYMS
DKFZp761G1421 antibody; KIAA1938 antibody; MRD5 antibody; Neuronal RasGAP antibody; OTTHUMP00000064825 antibody; p135 SynGAP antibody; Ras GTPase activating protein SynGAP antibody; Ras GTPase-activating protein SynGAP antibody; RASA 1 antibody; RASA 5 antibody; RASA1 antibody; RASA5 antibody; SYGP1_HUMAN antibody; Synaptic Ras GAP 1 antibody; Synaptic Ras GTPase activating protein 1 antibody; Synaptic Ras GTPase activating protein 1 homolog antibody; Synaptic Ras GTPase activating protein 135kDa antibody; Synaptic Ras GTPase activating protein antibody; Synaptic Ras GTPase-activating protein 1 antibody; Synaptic Ras-GAP 1 antibody; SYNGAP 1 antibody; SYNGAP1 antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. Phosphorylation by PLK2 promotes its activity (By similarity).
SUBCELLULAR LOCATION
Cytosol.
FUNCTION
Synaptic Ras GTPase-activating protein 1, also known as synaptic Ras-GAP 1 or SYNGAP1, is a protein that in humans is encoded by the SYNGAP1 gene. SYNGAP1 is a ras GTPase-activating protein that is critical for the development of cognition and proper synapse function. Mutations in humans can cause intellectual disability or epilepsy. SynGAP1 is a complex protein with several functions that may be regulated temporally via complex isoforms. A well-documented function of SynGAP1 involves NMDA receptor-mediated synaptic plasticity and membrane insertion of AMPA receptors through the suppression of upstream signaling pathways. However, SynGAP1 has also been shown to function cooperatively with Unc51.1 in axon formation. One way SynGAP1 affects these processes is through the MAP kinase signaling pathway by attenuation of Ras signalling. However, alternative splicing and multiple translational start sites have been shown to cause opposing effects, illustrating the importance of multiple functional domains that reside within the c- and n-termini. For example, the expression of an α1 or α2 c-terminal variant of SynGAP1 will either increase or decrease synaptic strength, respectively. Overall, SynGAP1 is essential for development and survival, which is evident as knockout mice die perinatally.