PRODUCT CODE: ER1802-68

SUFU Rabbit Polyclonal Antibody (ER1802-68)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of SUFU on 293 cell lysate using anti-SUFU antibody at 1/500 dilution.
  • Western blot analysis of SUFU on 293 cell lysate using anti-SUFU antibody at 1/500 dilution.
  • ICC staining SUFU in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUFU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SUFU antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SUFU antibody. Counter stained with hematoxylin.
Western blot analysis of SUFU on 293 cell lysate using anti-SUFU antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SUFU Rabbit Polyclonal Antibody (ER1802-68)

Immunogen

Recombinant protein within human sufu aa 33-260 / 484.

Host

Rabbit

Positive Control

293, SH-SY-5Y, A549, human kidney tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

SUFU

SYNONYMS

OTTHUMP00000020374 antibody; OTTHUMP00000020377 antibody; OTTHUMP00000020379 antibody; PRO1280 antibody; SU FU antibody; SU(F)U antibody; Su(fu) antibody; SUFU antibody; SUFU negative regulator of hedgehog signaling antibody; SUFU_HUMAN antibody; SUFUH antibody; SUFUXL antibody; Suppressor of fused homolog (Drosophila) antibody; Suppressor of fused homolog antibody

SEQUENCE SIMILARITIES

Belongs to the SUFU family.

TISSUE SPECIFICITY

Ubiquitous in adult tissues. Detected in osteoblasts of the perichondrium in the developing limb of 12-week old embryos. Isoform 1 is detected in fetal brain, lung, kidney and testis. Isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney.

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated at Lys-257 by the SCF(FBXL17) complex, leading to its subsequent degradation and allowing the release of GLI1 for proper hedgehog/smoothened signal transduction. Ubiquitination is impaired by phosphorylation at Ser-342, Ser-346, Ser-352 and Thr-353.; Phosphorylation at Ser-342, Ser-346, Ser-352 and Thr-353 prevents ubiquitination by the SCF(FBXL17) complex.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Su(fu) (for Suppressor-of-Fused) is a key negative regulator in the vertebrate Hedgehog signaling pathway. Su(fu) interacts with genes encoding proteins in this signal transduction pathway. In Drosophila, Intracellular transduction of the Hedgehog pathway involves the release of a large complex containing Su(fu). Su(fu) inhibits the activity of the transcription factor Gli1 and interacts with Gli2, Gli3 and the serine/threonine kinase Fused. Su(fu) is widely expressed in adult and embryonic tissues with higher expression in tissues patterned by hegdehog signaling. The Su(fu) gene locus maps to a region that is deleted in glioblastomas, prostate cancer, malignant melanoma and endometrial cancer.