PRODUCT CODE: ER40121

STAT6 Antibody (ER40121)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of STAT6 on different cell lysates using anti-STAT6 antibody at 1/1000 dilution.<br />
 Positive control: <br />
  Lane 1: Raji <br />
  Lane 2: Daudi <br />
  Lane 3: Hela <br />
  Lane 4: Ags <br />
  Lane 5: Jurkat
  • Western blot analysis of STAT6 on different cell lysates using anti-STAT6 antibody at 1/1000 dilution.<br />
 Positive control: <br />
  Lane 1: Raji <br />
  Lane 2: Daudi <br />
  Lane 3: Hela <br />
  Lane 4: Ags <br />
  Lane 5: Jurkat
  • ICC staining STAT6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-STAT6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-STAT6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-STAT6 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with ATAT6 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of STAT6 on different cell lysates using anti-STAT6 antibody at 1/1000 dilution.
Positive control:
Lane 1: Raji
Lane 2: Daudi
Lane 3: Hela
Lane 4: Ags
Lane 5: Jurkat

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

STAT6 Antibody (ER40121)

Immunogen

Peptide

Host

Rabbit

Positive Control

Jurkat, Raji, Daudi, Hela, Ags, human tosnsil tissue, human kidney tissue, human gastric carcinoma tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

94 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

STAT6

SYNONYMS

12S1644 antibody; D12S1644 antibody; IL 4 STAT antibody; IL-4 Stat antibody; IL4 STAT antibody; Interleukin 4 Induced antibody; Interleukin 4 Induced Transcription Factor IL4 STAT antibody; Signal transducer and activator of transcription 6 antibody; Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody; Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody; Signal transducer and activator of transcription 6, interleukin 4 induced antibody; STAT 6 antibody; STAT interleukin4 induced antibody; STAT, interleukin4 induced antibody; Stat6 antibody; STAT6_HUMAN antibody; STAT6B antibody; STAT6C antibody; Transcription factor IL 4 STAT antibody

SEQUENCE SIMILARITIES

Belongs to the transcription factor STAT family.

POST-TRANSLATIONAL MODIFICATION

Tyrosine phosphorylated on Tyr-641 following stimulation by IL4/interleukin-4. Tyrosine phosphorylated following stimulation by IL3/interleukin-3 (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling.; Mono-ADP-ribosylated by PARP14.

SUBCELLULAR LOCATION

Cytoplasm, nucleus.

FUNCTION

In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein STAT6 plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2), expression of cell surface markers, and class switch of immunoglobulins.