PRODUCT CODE: ER40121

STAT6 Antibody (ER40121)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of STAT6 on different cell lysates using anti-STAT6 antibody at 1/1000 dilution.<br />
Positive control: <br />
 Lane 1: Raji <br />
 Lane 2: Daudi <br />
 Lane 3: Hela <br />
 Lane 4: Ags <br />
 Lane 5: Jurkat
  • Western blot analysis of STAT6 on different cell lysates using anti-STAT6 antibody at 1/1000 dilution.<br />
Positive control: <br />
 Lane 1: Raji <br />
 Lane 2: Daudi <br />
 Lane 3: Hela <br />
 Lane 4: Ags <br />
 Lane 5: Jurkat
  • ICC staining STAT6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-STAT6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-STAT6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-STAT6 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with ATAT6 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of STAT6 on different cell lysates using anti-STAT6 antibody at 1/1000 dilution.
Positive control:
Lane 1: Raji
Lane 2: Daudi
Lane 3: Hela
Lane 4: Ags
Lane 5: Jurkat

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

STAT6 Antibody (ER40121)

Immunogen

Peptide

Host

Rabbit

Positive Control

Jurkat, Raji, Daudi, Hela, Ags, human tosnsil tissue, human kidney tissue, human gastric carcinoma tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

94 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Signal transducer and activator of transcription 6

GENE NAME

STAT6

SYNONYMS

STAT6

SEQUENCE SIMILARITIES

Belongs to the transcription factor STAT family.

POST-TRANSLATIONAL MODIFICATION

Tyrosine phosphorylated on Tyr-641 following stimulation by IL4/interleukin-4. Tyrosine phosphorylated following stimulation by IL3/interleukin-3 (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling.; Mono-ADP-ribosylated by PARP14.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.

FUNCTION

Carries out a dual signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.