Positive control:
Lane 1: Raji
Lane 2: Daudi
Lane 3: Hela
Lane 4: Ags
Lane 5: Jurkat
Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
STAT6 Antibody (ER40121)
Immunogen
Peptide
Host
Rabbit
Positive Control
Jurkat, Raji, Daudi, Hela, Ags, human tosnsil tissue, human kidney tissue, human gastric carcinoma tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
94 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
ICC
-
1:200
-
IHC-P
-
1:200
-
FC
-
1:100-1:200
TARGET
UNIPROT #
PROTEIN NAME
STAT6
SYNONYMS
12S1644 antibody; D12S1644 antibody; IL 4 STAT antibody; IL-4 Stat antibody; IL4 STAT antibody; Interleukin 4 Induced antibody; Interleukin 4 Induced Transcription Factor IL4 STAT antibody; Signal transducer and activator of transcription 6 antibody; Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody; Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody; Signal transducer and activator of transcription 6, interleukin 4 induced antibody; STAT 6 antibody; STAT interleukin4 induced antibody; STAT, interleukin4 induced antibody; Stat6 antibody; STAT6_HUMAN antibody; STAT6B antibody; STAT6C antibody; Transcription factor IL 4 STAT antibody
SEQUENCE SIMILARITIES
Belongs to the transcription factor STAT family.
POST-TRANSLATIONAL MODIFICATION
Tyrosine phosphorylated on Tyr-641 following stimulation by IL4/interleukin-4. Tyrosine phosphorylated following stimulation by IL3/interleukin-3 (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling.; Mono-ADP-ribosylated by PARP14.
SUBCELLULAR LOCATION
Cytoplasm, nucleus.
FUNCTION
In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein STAT6 plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2), expression of cell surface markers, and class switch of immunoglobulins.