PRODUCT CODE: ER1706-49

SOD1 Antibody (ER1706-49)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SOD1 on A549 cell lysate using anti-SOD1 antibody at 1/500 dilution.
  • Western blot analysis of SOD1 on A549 cell lysate using anti-SOD1 antibody at 1/500 dilution.
  • ICC staining SOD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SOD1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of MCF-7 cells with SOD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of SOD1 on A549 cell lysate using anti-SOD1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SOD1 Antibody (ER1706-49)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, HepG2, LOVO, rat testis tissue, human liver tissue, human kidney tissue, mouse brain tissue,MCF-7.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

16 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Superoxide dismutase [Cu-Zn]

GENE NAME

SOD1

SYNONYMS

hSod1, SOD1

SEQUENCE SIMILARITIES

Belongs to the Cu-Zn superoxide dismutase family.

POST-TRANSLATIONAL MODIFICATION

Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.; The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.; Palmitoylation helps nuclear targeting and decreases catalytic activity.; Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity.

SUBCELLULAR LOCATION

Cytoplasm. Mitochondrion. Nucleus. Note=Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.

FUNCTION

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.