PRODUCT CODE: ER1706-93

SHP-2 Antibody (ER1706-93)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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ICC staining SHP2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SHP2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SHP2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SHP2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-SHP2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cacner tissue using anti-SHP2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SHP2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SHP2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with SHP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
ICC staining SHP2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SHP-2 Antibody (ER1706-93)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, LOVO, MCF-7, rat skeletal muscle tissue, human colon cancer tissue, human fetal skeletal muscle tissue, mouse brain tissue, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SHP-2

SYNONYMS

BPTP3 antibody; CFC antibody; JMML antibody; METCDS antibody; MGC14433 antibody; NS1 antibody; OTTHUMP00000166107 antibody; OTTHUMP00000166108 antibody; Protein tyrosine phosphatase 2 antibody; Protein tyrosine phosphatase 2C antibody; Protein tyrosine phosphatase non receptor type 11 antibody; Protein-tyrosine phosphatase 1D antibody; Protein-tyrosine phosphatase 2C antibody; PTN11_HUMAN antibody; PTP-1D antibody; PTP-2C antibody; PTP1D antibody; PTP2C antibody; PTPN11 antibody; SAP2 antibody; SH-PTP2 antibody; SH-PTP3 antibody; SH2 domain containing protein tyrosine phosphatase 2 antibody; SHP 2 antibody; SHP-2 antibody; Shp2 antibody; SHPTP2 antibody; SHPTP3 antibody; Syp antibody; Tyrosine-protein phosphatase non-receptor type 11 antibody

SEQUENCE SIMILARITIES

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

TISSUE SPECIFICITY

Widely expressed, with highest levels in heart, brain, and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog.