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PRODUCT CODE: ER1802-34

SFRP1 Antibody (ER1802-34)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of SFRP1 on SH-SY-5Y cells lysates using anti-SFRP1 antibody at 1/500 dilution.
  • Western blot analysis of SFRP1 on SH-SY-5Y cells lysates using anti-SFRP1 antibody at 1/500 dilution.
  • ICC staining SFRP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SFRP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with SFRP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SFRP1 on SH-SY-5Y cells lysates using anti-SFRP1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SFRP1 Antibody (ER1802-34)

Immunogen

Peptide

Host

Rabbit

Positive Control

A549, SH-SY-5Y, rat testis tissue, human colon cancer tissue, human breast tissue, human placenta tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

35 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q8N474

PROTEIN NAME

SFRP1

SYNONYMS

Frizzled related protein 1 antibody; FRP 1 antibody; FRP antibody; FRP-1 antibody; FRP1 antibody; FrzA antibody; SARP 2 antibody; SARP-2 antibody; SARP2 antibody; Secreted apoptosis related protein 2 antibody; Secreted apoptosis-related protein 2 antibody; Secreted frizzled related protein 1 antibody; Secreted frizzled related protein antibody; Secreted frizzled-related protein 1 antibody; SFRP 1 antibody; sFRP-1 antibody; SFRP1 antibody; SFRP1_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the secreted frizzled-related protein (sFRP) family.

TISSUE SPECIFICITY

Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription.