PRODUCT CODE: ER1802-92

sfGFP antibdody Antibody (ER1802-92)

Applications

  • WB

REACTIVITY

Western blot analysis of sfGFP on fusion protein using anti-sfGFP antibody at 1/1,000 dilution.
  • Western blot analysis of sfGFP on fusion protein using anti-sfGFP antibody at 1/1,000 dilution.
Western blot analysis of sfGFP on fusion protein using anti-sfGFP antibody at 1/1,000 dilution.

Applications

  • WB

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

sfGFP antibdody Antibody (ER1802-92)

Immunogen

Synthetic peptide rdhmvlheyvnaagitc linked to klh.

Host

Rabbit

Positive Control

GFP11-tagged fusion protein.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

TARGET

PROTEIN NAME

Anti-sfGFP antibdody

FUNCTION

Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.