PRODUCT CODE: ER1803-06

SERPINC1 Antibody (ER1803-06)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SERPINC1 on different lysates using anti-SERPINC1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2 <br />
 Lane 2: Mouse smooth muscle<br />
 Lane 3: Mouse lung<br />
  • Western blot analysis of SERPINC1 on different lysates using anti-SERPINC1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2 <br />
 Lane 2: Mouse smooth muscle<br />
 Lane 3: Mouse lung<br />
  • ICC staining SERPINC1 in H22 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SERPINC1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with SERPINC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SERPINC1 on different lysates using anti-SERPINC1 antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Mouse smooth muscle
Lane 3: Mouse lung

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SERPINC1 Antibody (ER1803-06)

Immunogen

Recombinant protein within human serpinc1 aa 150-400.

Host

Rabbit

Positive Control

HepG2, mouse smooth muscle tissue, mouse lung tissue, H22, rat kidney tissue, human liver tissue, human small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1

  • 1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Antithrombin-III

GENE NAME

SERPINC1

SYNONYMS

ATIII, SERPINC1, AT3

SEQUENCE SIMILARITIES

Belongs to the serpin family.

TISSUE SPECIFICITY

Found in plasma.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by FAM20C in the extracellular medium.

SUBCELLULAR LOCATION

Secreted, extracellular space.

FUNCTION

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.