PRODUCT CODE: ER1803-63

SDHB Antibody (ER1803-63)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SDHB on different cell lysate using anti-SDHB antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: human liver<br />
 Lane 2: mouse liver<br />
 Lane 3: rat liver<br />
 Lane 4: rat liver, preincubated with the immunization protein.<br />
  • Western blot analysis of SDHB on different cell lysate using anti-SDHB antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: human liver<br />
 Lane 2: mouse liver<br />
 Lane 3: rat liver<br />
 Lane 4: rat liver, preincubated with the immunization protein.<br />
  • ICC staining SDHB in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SDHB in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SDHB on different cell lysate using anti-SDHB antibody at 1/1,000 dilution.
Positive control:
Lane 1: human liver
Lane 2: mouse liver
Lane 3: rat liver
Lane 4: rat liver, preincubated with the immunization protein.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SDHB Antibody (ER1803-63)

Immunogen

Recombinant protein within human sdhb aa 100-300.

Host

Rabbit

Positive Control

Rat liver tissue, human liver tissue, mouse liver tissue, 293T, HT-29, rat heart tissue, human kidney tissue, human skin tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

GENE NAME

SDHB

SYNONYMS

Ip, SDHB, SDH, SDH1

SEQUENCE SIMILARITIES

Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

SUBCELLULAR LOCATION

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

FUNCTION

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).