PRODUCT CODE: ER1803-61

SCNN1G Antibody (ER1803-61)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SCNN1G on A431 cell lysate using anti-SCNN1G antibody at 1/1,000 dilution.
  • Western blot analysis of SCNN1G on A431 cell lysate using anti-SCNN1G antibody at 1/1,000 dilution.
  • ICC staining SCNN1G in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SCNN1G in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SCNN1G antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SCNN1G antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with SCNN1G antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SCNN1G on A431 cell lysate using anti-SCNN1G antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SCNN1G Antibody (ER1803-61)

Immunogen

Recombinant protein within human scnn1g aa 250-350.

Host

Rabbit

Positive Control

A431, 293T, rat kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

74 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Amiloride-sensitive sodium channel subunit gamma

GENE NAME

SCNN1G

SYNONYMS

ENaCG, Gamma-ENaC, SCNN1G

SEQUENCE SIMILARITIES

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily.

TISSUE SPECIFICITY

Expressed in kidney (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.; Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.; ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.

SUBCELLULAR LOCATION

Apical cell membrane; Multi-pass membrane protein.

FUNCTION

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.