Applications
-
WB
-
ICC
REACTIVITY
-
Mouse
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
SCA-1 Antibody (0804-10)
Immunogen
Peptide
Host
Rabbit
Positive Control
Mouse liver
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Immunogen affinity purified
MOLECULAR WEIGHT
18-20kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
TARGET
UNIPROT #
PROTEIN NAME
SCA-1
SYNONYMS
Ly-6A.2/Ly-6E. antibody; Ly-6A.2/Ly-6E.1 antibody; Ly6a antibody; LY6A_MOUSE antibody; Ly6al antibody; Lymphocyte antigen 6A-2/6E-1 antibody; Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody; SCA-1 antibody; Stem cell antigen 1 antibody; T cell activating protein antibody; T-cell-activating protein antibody; TAP antibody
TISSUE SPECIFICITY
Widely expressed.
POST-TRANSLATIONAL MODIFICATION
O-glycosylated. Not N-glycosylated.; Not phosphorylated.
SUBCELLULAR LOCATION
Cell membrane
FUNCTION
Sca-1, an 18 kDa phosphatidylinositol-anchored protein, is a member of the Ly-6 antigen family. Sca-1 is the most recognized HSC marker in mice with both Ly-6 haplotypes as it is expressed on multipotent HSCs. Sca-1+ HSCs can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen within the adult animal. An anti-Sca-1 Ab is frequently used in combination with negative selection for expression of a number of cell surface markers characteristic of differentiated cells of hematolymphoid lineages (Lin-) to identify and isolate murine HSCs.