PRODUCT CODE: ER1802-24

SATB1 Antibody (ER1802-24)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of SATB1 on mouse thymus tissue lysate using anti-SATB1 antibody at 1/500 dilution.
  • Western blot analysis of SATB1 on mouse thymus tissue lysate using anti-SATB1 antibody at 1/500 dilution.
  • ICC staining SATB1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Jurkat cells with SATB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of SATB1 on mouse thymus tissue lysate using anti-SATB1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SATB1 Antibody (ER1802-24)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse thymus tissue lysate,SH-SY-5Y, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

Predicted band size 86 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SATB1

SYNONYMS

DNA binding protein SATB1 antibody; DNA-binding protein SATB1 antibody; SATB homeobox 1 antibody; SATB1 antibody; SATB1_HUMAN antibody; Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody; Special AT rich sequence binding protein 1 antibody; Special AT-rich sequence-binding protein 1 antibody

SEQUENCE SIMILARITIES

Belongs to the CUT homeobox family.

TISSUE SPECIFICITY

Expressed predominantly in thymus.

POST-TRANSLATIONAL MODIFICATION

Sumoylated. Sumoylation promotes cleavage by caspases.; Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.; Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms cannot dimerize and lose transcription regulation function because of impaired DNA and chromatin association.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The homeoproteins CCAAT displacement protein (CDP) and special AT-rich sequence binding protein 1 (SATB1) are transcriptional repressors of many cellular genes, and they participate in cell development and cell type differentiation. SATB1 is expressed primarily in thymocytes, and, like CDP, it also contains a distinct homeobox DNA-binding domain that is essential for DNA binding. SATB1 and CDP interact through these homeodomains and synergistically function as mediators of gene expression. SATB1 contains an additional domain that has a higher affinity for DNA and specifically facilitates the direct association between SATB1 and the nuclear matrix attachment regions (MARs) of DNA. MARs are specific DNA sequences that bind to the nuclear matrix and form the base of chromosomal loops that organize the chromosomes and regulate DNA transcription and replication within the nucleus. The association of SATB1 with the core unwinding element within the base-unpairing region of MARs requires both the MAR and homeobox binding domains of SATB1.