PRODUCT CODE: EM1902-21

SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody (EM1902-21)

Applications

  • ELISA

  • WB

REACTIVITY

  • SARS-CoV-2

The binding activity of EM1902-21 with SARS-CoV-2 Recombinant Nucleocapsid protein.<br />
 Immobilized SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (EM1902-21) for 1 hour at 25℃. The EC50 of EM1902-21 is 271.3 ng/ml.
  • The binding activity of EM1902-21 with SARS-CoV-2 Recombinant Nucleocapsid protein.<br />
 Immobilized SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (EM1902-21) for 1 hour at 25℃. The EC50 of EM1902-21 is 271.3 ng/ml.
  • Western blot analysis of EM1902-21 with N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein (1) and Inactivated SARS-CoV-2 lysate (2) at 20ng.<br />
 Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
The binding activity of EM1902-21 with SARS-CoV-2 Recombinant Nucleocapsid protein.
Immobilized SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (EM1902-21) for 1 hour at 25℃. The EC50 of EM1902-21 is 271.3 ng/ml.

Applications

  • ELISA

  • WB

REACTIVITY

  • SARS-CoV-2

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody (EM1902-21)

Immunogen

Recombinant sars-cov-2 nucleocapsid protein.

Host

Mouse

Positive Control

ELISA: Recombinant SARS-CoV-2 Nucleocapsid Protein. WB: N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein and inactivated native SARS-CoV-2 lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A4D12

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Concentration

1 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

46 kDa

Isotype

IgG1

APPLICATION DILUTION

  • ELISA

  • assay-dependent

  • WB

  • 1:500-1:5,000

TARGET

PROTEIN NAME

SARS-CoV-2 Nucleocapsid Protein

SYNONYMS

SARS-CoV-2 Nucleocapsid Protein antibody; SARS-CoV-2 NP antibody; nucleocapsid protein antibody; novel coronavirus N Protein antibody; novel coronavirus Nucleocapsid Protein antibody; 2019-nCoV Nucleoprotein antibody; 2019-nCoV N antibody; 2019nCoV N antibody; 2019-nCoV N Protein antibody; 2019 peak N Protein antibody; 2019-nCoV nucleocapsid protein antibody

SEQUENCE SIMILARITIES

Belongs to the betacoronavirus nucleocapsid protein family.

POST-TRANSLATIONAL MODIFICATION

ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.; Phosphorylated on serine and threonine residues.

SUBCELLULAR LOCATION

Virion, Host endoplasmic reticulum-Golgi intermediate compartment, Host Golgi apparatus.

FUNCTION

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.