PRODUCT CODE: ER1803-19

RYR1 Antibody (ER1803-19)

Applications

  • Dot-blot

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Dot blot analysis of anti-RYR1 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR1 antibody was diluted with 1/500.
  • Dot blot analysis of anti-RYR1 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR1 antibody was diluted with 1/500.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RYR1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RYR1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SiHa cells with RYR1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Dot blot analysis of anti-RYR1 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR1 antibody was diluted with 1/500.

Applications

  • Dot-blot

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

RYR1 Antibody (ER1803-19)

Immunogen

Synthetic peptide within human ryr1 aa 1350-1400.

Host

Rabbit

Positive Control

Human fetal skeletal muscle tissue, mouse cerebellum tissue, SiHa.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

565 kDa

Isotype

IgG

APPLICATION DILUTION

  • Dot-blot

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

RYR1

SYNONYMS

Arrhythmogenic right ventricular dysplasia 2 antibody; ARVC2 antibody; ARVD2 antibody; Brain ryanodine receptor calcium release channel antibody; Brain type ryanodine receptor antibody; Cardiac muscle ryanodine receptor calcium release channel antibody; Cardiac muscle type ryanodine receptor antibody; CCO antibody; Central core disease of muscle antibody; HBRR antibody; hRYR 2 antibody; hRYR2 antibody; MHS antibody; MHS1 antibody; Ryanodine receptor 1 (skeletal) antibody; Ryanodine receptor 1 antibody; Ryanodine receptor 2 (cardiac) antibody; Ryanodine receptor 2 antibody; Ryanodine receptor 3 antibody; Ryanodine receptor type1 antibody; RYDR antibody; RYR 1 antibody; RYR 2 antibody; RYR 3 antibody; RYR antibody; RYR-1 antibody; RyR1 antibody; RYR1_HUMAN antibody; RYR2 antibody; RYR3 antibody; Sarcoplasmic reticulum calcium release channel antibody; Skeletal muscle calcium release channel antibody; Skeletal muscle ryanodine receptor antibody; Skeletal muscle type ryanodine receptor antibody; Skeletal muscle-type ryanodine receptor antibody; SKRR antibody; Type 1 like ryanodine receptor antibody; Type 1 ryanodine receptor antibody; VTSIP antibody

SEQUENCE SIMILARITIES

Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.

TISSUE SPECIFICITY

Skeletal muscle and brain (cerebellum and hippocampus).

POST-TRANSLATIONAL MODIFICATION

Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843.; Activated by reversible S-nitrosylation (By similarity). Repeated very high-level exercise increases S-nitrosylation.

SUBCELLULAR LOCATION

Sarcoplasmic reticulum membrane.

FUNCTION

Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca2+ leaking into the cytoplasm. Can also mediate the release of Ca2+ from intracellular stores in neurons, and may thereby promote prolonged Ca2+ signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.