PRODUCT CODE: ER1802-78

RUNX2 Antibody (ER1802-78)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

Western blot analysis of RUNX2 on HL-60 cell lysate using anti-RUNX2 antibody at 1/500 dilution.
  • Western blot analysis of RUNX2 on HL-60 cell lysate using anti-RUNX2 antibody at 1/500 dilution.
  • ICC staining RUNX2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.
Western blot analysis of RUNX2 on HL-60 cell lysate using anti-RUNX2 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

RUNX2 Antibody (ER1802-78)

Immunogen

Synthetic peptide of c-terminal human runx2.

Host

Rabbit

Positive Control

HL-60, A431, human tonsil tissue, human colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

56.6 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Runt-related transcription factor 2

GENE NAME

RUNX2

SYNONYMS

CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A, RUNX2, AML3, CBFA1, OSF2, PEBP2A

TISSUE SPECIFICITY

Specifically expressed in osteoblasts.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated; probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation (By similarity). Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.