PRODUCT CODE: ET7107-51

RPA70 Recombinant Rabbit Monoclonal Antibody [JB75-32] (ET7107-51)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.
  • Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.
  • ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RPA70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RPA70 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of 293T cells with RPA70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

RPA70 Recombinant Rabbit Monoclonal Antibody [JB75-32] (ET7107-51)

Immunogen

Recombinant protein corresponding to human rpa70 aa 1-100.

Host

Rabbit

Positive Control

Siha, 293T, A549, LOVO.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB75-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

RPA70

SYNONYMS

Dmrpa1 antibody; Drosophila Replication Protein A antibody; DRPA antibody; HSSB antibody; Human single stranded DNA binding protein antibody; MST075 antibody; MSTP075 antibody; p70 antibody; REPA1 antibody; Replication factor A antibody; Replication factor A protein 1 antibody; Replication protein A 70 kDa DNA-binding subunit antibody; Replication protein A 70kDa DNA binding subunit antibody; Replication protein A1 70kDa antibody; Replication protein A1 antibody; RF A antibody; RF-A protein 1 antibody; RFA antibody; RFA1_HUMAN antibody; RP A antibody; RP-A p70 antibody; RPA 70 antibody; RPA antibody; rpa1 antibody; Single stranded binding protein 70 antibody; Single-stranded DNA-binding protein antibody

SEQUENCE SIMILARITIES

Belongs to the replication factor A protein 1 family.

POST-TRANSLATIONAL MODIFICATION

DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination.; Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Desumoylated by SENP6.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner.