PRODUCT CODE: ER40122

ROCK1 Antibody (ER40122)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of ROCK1 on different cell lysates using anti-ROCK1 antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: NIH/3T3 <br />
Lane 2: Jurkat <br />
Lane 3: Hela
  • Western blot analysis of ROCK1 on different cell lysates using anti-ROCK1 antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: NIH/3T3 <br />
Lane 2: Jurkat <br />
Lane 3: Hela
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ROCK1 antibody. Counter stained with hematoxylin.
Western blot analysis of ROCK1 on different cell lysates using anti-ROCK1 antibody at 1/1000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: Jurkat
Lane 3: Hela

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ROCK1 Antibody (ER40122)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, NIH/3T3, Jurkat, human colon cancer tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

158 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • ICC

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Rho-associated protein kinase 1

GENE NAME

ROCK1

SYNONYMS

ROCK-I,p160ROCK

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

TISSUE SPECIFICITY

Detected in blood platelets.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated on serine and threonine residues.; Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

SUBCELLULAR LOCATION

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Note=Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.

FUNCTION

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity). Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity).